According to reports, upcoming DLC pack will support the ability to have a second save file for the game although only for the new Master Mode.
This was how it was explained in the comments:
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For anyone wondering, Master Mode is really just a mode - it can be turned on and off on the same save file, Treehouse's Audrey confirmed this in a stream during E3. It's odd that they're allowing us a different save for that (even if it's the logical choice), but suppose most people would like to keep their Master and regular adventures separate, or at least that's what I'll be doing.
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Whenever the mode is turned on, it writes to its own save file and when turned off, the regular save file gets used again.
https://www.destructoid.com/you-ll-h...--443629.phtml